This study was aimed to investigate the impact of rotation sampling on feature parameters of texture images of Chinese herbal medicine. Four Chinese herb medicine with various shape and texture feature were taken as research materials. Images of complete and incomplete herbal medicine were collected respectively after different rotation angles. The 26 parameters were extracted by gray-level co-occurrence matrix and grayscale gradient matrix. The impact of rotation sampling on feature parameters was investigated through analysis of variation tendency and range of 26 parameters. The results showed that if the Chinese herbal medicine was complete, the 26 parameters were not impacted by the rotation angle, whereas the 26 parameters were impacted by the rotation angle and the impact will be more obvious when the shape of Chinese herb medicine was irregular. It was concluded that in order to get a high quality of images and construct a well identification model based on the parameter of texture features, we must consider the impact of rotation angle on the parameters to Chinese herbal medicine with various shapes and texture features.