This study was aimed to explore the impact of integrality of Chinese herbal medicine on parameters of texture feature in transverse section images. Three Chinese herbal medicine of Semen Arecae, Radix et Rhizoma Rhei and Radix Atractylodis Macrocephalae with different texture features were taken as research materials. Parts of Chinese herbal medicine were cut off from the whole by equal proportions. The 26 parameters were extracted by graylevel co-occurrence matrix and grayscale gradient matrix. The similarities and differences of 26 parameters of texture feature in the parts and whole, rectangular and fan-shaped Chinese herbal medicine were compared. The results showed that parameters of Semen Arecae and Radix et Rhizoma Rhei with radial or annular texture had better consistency in whole and fan-shaped parts. Parameters of Radix Atractylodis Macrocephalae with irregular texture feature were approximately the same in the whole and rectangular parts. It was concluded that whether parameters of texture features in parts Chinese herbal medicine can present the whole were related to its texture feature and the shape of the parts. This study provided the basis for collection of Chinese herbal medicine when sampling images. It also laid a foundation for the extraction of accurate parameter of texture feature.