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基于Th细胞平衡探讨“宣降清”三法对RSV感染幼龄大鼠 T-bet、GATA3表达的影响
Study on Effect of “Xuan-Jiang-Qing” Theory on Expression of T-bet And GATA3 Among Young Rats Infected with RSV Based on Th Cell Balance
  
DOI:
中文关键词:  宣降清  RSV  大鼠  Th1/Th2
英文关键词:Keywords: Xuan-Jiang-Qing, RSV, rat, Th1/Th2
基金项目:
作者单位
崔振泽1,徐 超1,黄 燕 1.大连市儿童医院 116000 
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中文摘要:
      摘 要: 目的:观察定喘汤“宣降清”三法对呼吸道合胞病毒(Respiratorysyncytical virus,RSV)感染幼龄大鼠肺组织内T-bet,GATA3,和血清中IFN-γ,IL-4的表达的影响,并进一步阐明该方药对调节Th1/Th2免疫平衡的作用机制。方法:选健康幼龄Wistar大鼠54只随机分为6组,即正常组,RSV模型组,定喘汤组,分解剂组(包括宣,降,清三组),每组各9只。除正常组外,余各组用RSV(Long珠)病毒予大鼠滴鼻造模,滴鼻2h后各干预治疗组分别予相应的药物灌胃,正常组和RSV模型组均以等体积的生理盐水灌胃。采用Western Blot法检测各组大鼠肺组织中T-bet和GATA3 的蛋白表达水平,ELISA法检测各组大鼠血清中INF-γ 和IL-4细胞因子的含量。结果:1,定喘汤及各分解剂组均可不同程度的调节RSV感染后大鼠的一般状态和降低RSV感染后的肺指数(P<0.05);2,RSV模型组和各干预组大鼠较正常组比较肺组织中T-bet蛋白表达均降低,GATA3蛋白表达均升高且具有显著性差异(P<0.05),定喘汤及宣法组与RSV模型组比较均可上调T-bet蛋白表达及下调GATA3蛋白表达且具有显著性差异(P<0.05);3,RSV模型组大鼠血清中INF-γ含量明显降低,IL-4含量升高与正常组比较均具有显著性差异(P<0.05),与模型组比较,定喘汤组可上调INF-γ含量,各干预组药物均可下调IL-4含量且具有统计学意义(P<0.05)。结论:定喘汤“宣降清”三法对于RSV感染幼龄大鼠后体内T-bet及INF-γ细胞因子具有明显升高作用,而对GATA3及 IL-4细胞因子则有明显抑制效果,其具有调节Th1/Th2细胞免疫平衡能力,为定喘汤防治RSV感染有效性的重要机制之一
英文摘要:
      Abstract: This study was aimed to observe the method of “Xuan-Jiang-Qing” of Ding-Chuan (DC) decoction on the expression of T-bet and GATA3 of lung tissues, IFN-γ and IL-4 in serum of young rats infected with respiratory syncytial virus (RSV), in order to further explore the mechanism of Th1 / Th2 immune balance. A total of 54 ealthy young Wistar rats were randomly divided into 6 groups, which were the normal group, RSV model group, DC decoction group, ascending (Xuan) therapy group, descending (Jiang) therapy group, pyretic clearing (Qing) therapy group, with 9 rats in each group. Except the normal group, rats in other groups were with RSV (Long bead) virus to establish the animal model. Two hours after intranasal medication, intragastric administration of corresponding medication was given to each intervention group. And equal volume of normal saline was given to the normal group and the RSV model group. Levels of T-bet and GATA3 protein in lung tissues were detected by Western blotting. Levels of INF-γ and IL-4 cytokines in serum were detected by ELISA. The results showed that DC decoction and each decomposing agent group can adjust the general condition of RSV infection and reduce the lung index after RSV infection (P < 0.05). Compared with the normal group, the expression of T-bet protein in lung tissues was decreased and the expression of GATA3 protein was significantly higher in the RSV model group and each intervention group with significant difference (P < 0.05). The expression of T-bet protein was up-regulated and the expression of GATA3 protein was down-regulated in DC decoction and ascending (Xuan) therapy group compared to the RSV model group with significant difference (P < 0.05). Compared with the normal group, the content of INF-γ in serum of RSV model group was significantly decreased, and the content of IL-4 was increased with significant difference (P < 0.05). Compared with the model group, DC decoction group can increase the content of INF-γ, and each intervention group can reduce the IL-4 content with statistical significance (P < 0.05). It was concluded that the method of “Xuan-Jiang-Qing” of DC decoction can obviously increase T-bet and INF-γ cytokines, and inhibit GATA3 and IL-4 cytokines among RSV infected young rats. It can regulate Th1 / Th2 cell immune balance. It is one of the important effectiveness mechanisms to prevent and treat RSV infection.
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