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高效液相色谱法同时测定白虎加桂枝汤中5个有效指标性成分的含量 |
Simultaneous Determination of Five Components in Baihu And Guizhi Decoction by HPLC |
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DOI: |
中文关键词: 关键词:白虎汤 芒果苷 甘草苷 肉桂酸 |
英文关键词:Keywords: Baihu decoction, mangiferin, liquiritin, cinnamic acid |
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摘要点击次数: 215 |
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中文摘要: |
摘 要:目的:建立白虎加桂枝汤中芒果苷、新芒果苷、甘草酸、甘草苷、肉桂酸5个有效指标性成分的含量测定方法,为白虎加桂枝汤的颗粒研发奠定基础。方法:采用HPLC-PDA双波长同时测定,Waters xBridge BEH C18(4.6 mm×250 μm,5 μm)色谱柱,流动相乙腈-0.01%甲酸水,梯度洗脱,流速1 mL?min-1,柱温30℃,检测波长255 nm、280 nm。结果:70 min内芒果苷、新芒果苷、甘草酸、甘草苷、肉桂酸线性范围分别为66-1976 μg(r=0.999 3),70-3487 μg(r=0.999 7),30-913 μg(r=0.999 5),35-1734 μg(r=0.999 5),0.6-187 μg(r=0.999 8),加样回收率平均范围95.29%-100.17%。结论:建立了白虎加桂枝汤中5个有效指标性成分的含量测定方法,该方法简捷、稳定、准确,可为白虎加桂枝汤及其复方颗粒研制的质量控制提供评价方法。 |
英文摘要: |
Abstract: This study was aimed to develop an HPLC method for simultaneous determination of five active components in Baihu and Guizhi decoction. Simultaneous determination of mangiferin, neomangiferin, glycyrrhizic acid, liquiritin, and cinnamic acid in Baihu and Guizhi decoction were conducted by HPLC-PDA under multiple UV wavelengths. An Waters xBridge BEH C18 Column (4.6 mm × 250 μm, 5 μm) was used. The mobile phase was acetonitrile-0.01% formic acid solution. The flow rate was 1 mL?min-1. The column temperature was kept at 30°C. The detection wavelength was set at 255 nm and 280 nm. The results showed that the linear range of mangiferin, neomangiferin, glycyrrhizic acid, liquiritin, and cinnamic acid was 66-1976 μg (r = 0.9993), 70-3487 μg (r = 0.9997), 30-913 μg (r = 0.9995), 35-1734 μg (r = 0.9995), 0.6-187 μg (r = 0.9998) in 70 min, respectively. The average recovery was between 95.29% and 100.17%. It was concluded that the method was convenient, stable, reliable and accurate for simultaneous determination of the five components in Baihu and Guizhi decoction. It provided an evaluation method for the quality control of Baihu and Guizhi decoction and the research o its granules. |
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